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48 / 96 Wells Cga Human Elisa Kit Sandwich Highly Sensitive With Oem Service

Shanghai Korain Biotech Co., Ltd
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    Buy cheap 48 / 96 Wells Cga Human Elisa Kit Sandwich Highly Sensitive With Oem Service from wholesalers
     
    Buy cheap 48 / 96 Wells Cga Human Elisa Kit Sandwich Highly Sensitive With Oem Service from wholesalers
    • Buy cheap 48 / 96 Wells Cga Human Elisa Kit Sandwich Highly Sensitive With Oem Service from wholesalers

    48 / 96 Wells Cga Human Elisa Kit Sandwich Highly Sensitive With Oem Service

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    Brand Name : BT Lab
    Model Number : Cat.No E1730Hu
    Certification : CE, ISO9001:2005, MSDS
    Price : Negotiation
    Supply Ability : Western Union, T/T
    Delivery Time : 1-3 business days, bulk order within one week
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    48 / 96 Wells Cga Human Elisa Kit Sandwich Highly Sensitive With Oem Service

    48 Wells 96 Wells Human ELISA Kit Sandwich Human High Sensitive CGA ELISA Kit

    Cat.No E1730Hu
    Size: 96 wells
    *This product is for research use only, not for use in diagnosis procedures. It’s highly recommend to read this instruction entirely before use.

    Intended Use
    This sandwich kit is for the accurate quantitative detection of human Chromogranin A (also known as CGA) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

    Assay Principle
    This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human CGA antibody. CGA present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human CGA Antibody is added and binds to CGA in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CGA antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human CGA. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

    Precautions

    • Prior to use, the kit and sample should be warmed naturally to room temperature 30 minutes.
    • This instruction must be strictly followed in the experiment.
    • Once the desired number of strips has been removed, immediately reseal the bag to protect the remain from deterioration. Cover all reagents when not in use.
    • Make sure pipetting order and rate of addition from well-to-well when pipetting reagents.
    • Pipette tips and plate sealer in hand should be clean and disposable to avoid cross-contamination.
    • Avoid using the reagents from different batches together.
    • Substrate solution B is sensitive to light, don’t expose substrate solution B to light for a long time.
    • Stop solution contains acid. Please wear eye, hand and skin protection when using this material. Avoid contact of skin or mucous membranes with kit reagent.
    • The kit should not be used beyond the expiration date.


    Specimen Collection
    Serum Allow serum to clot for 10-20 minutes at room temperature. Centrifuge at 2000-3000 RPM for 20 minutes.

    Plasma Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 2000-3000 RPM at 2 - 8°C within 30 minutes of collection.

    Urine Collect by sterile tube. Centrifuge at 2000-3000 RPM for approximately 20 minutes. When collecting pleuroperitoneal fluid and cerebrospinal fluid, please follow the procedures above-mentioned.

    Cell Culture Supernatant Collect by sterile tubes when examining secrete components. Centrifuge at 2000-3000 RPM for approximately 20 minutes. Collect the supernatants carefully. When examining the components within the cell, use PBS (pH 7.2-7.4) to dilute cell suspension to the cell concentration of approximately 1 million/ml. Damage cells through repeated freeze-thaw cycles to let out the inside components. Centrifuge at 2000-3000 RPM for approximately 20 minutes.

    Tissue Rinse tissues in PBS (pH 7.4) to remove excess blood thoroughly and weigh before homogenization. Mince tissues and homogenize them in PBS (pH7.4) with a glass homogenizer on ice. Thaw at 2-8°C or freeze at -20°C. Centrifuge at 2000-3000 RPM for approximately 20 minutes.

    *Sample can't be diluted with this kit. Owing to the the material we use to prepare the kit, the sample matrix interference may falsely depress the specificity and accuracy of the assay.

    Reagent Preparation
    All reagents should be brought to room temperature before use.
    Standard Reconstitute the 120μl of the standard (9600ng/L) with 120μl of standard diluent to generate a 4800ng/L standard stock solution. Allow the standard to sit for 15 mins with gentle agitation prior to making dilutions. Prepare duplicate standard points by serially diluting the standard stock solution (4800ng/L) 1:2 with standard diluent to produce 2400ng/L, 1200ng/L, 600ng/L and 300ng/L solutions. Standard diluent serves as the zero standard(0 ng/L). Any remaining solution should be frozen at -20°C and used within one month. Dilution of standard solutions suggested are as follows:

    4800ng/LStandard No.5120μl Original Standard + 120μl Standard Diluent
    2400ng/LStandard No.4120μl Standard No.5 + 120μl Standard Diluent
    1200ng/LStandard No.3120μl Standard No.4 + 120μl Standard Diluent
    600ng/LStandard No.2120μl Standard No.3 + 120μl Standard Diluent
    300ng/LStandard No.1120μl Standard No.2 + 120μl Standard Diluent

    Standard ConcentrationStandard No.5Standard No.4Standard No.3Standard No.2Standard No.1
    9600ng/L4800ng/L2400ng/L1200ng/L600ng/L300ng/L


    Wash Buffer Dilute 20ml of Wash Buffer Concentrate 30x into deionized or distilled water to yield 500 ml of 1x Wash Buffer. If crystals have formed in the concentrate, mix gently until the crystals have completely dissolved.

    Summary
    1. Prepare all reagents, samples and standards.
    2. Add sample and ELISA reagent into each well. Incubate for 1 hour at 37°C.
    3. Wash the plate 5 times.
    4. Add substrate solution A and B. Incubate for 10 minutes at 37°C.
    5. Add stop solution and color develops.
    6. Read the OD value within 10 minutes.

    Referances
    "Chromogranin A and chromogranin B in noninvasive and invasive breast carcinoma."
    Kimura N., Yoshida R., Shiraishi S., Pilichowska M., Ohuchi N.
    Endocr. Pathol. 13:117-122(2002)

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