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Cystatin C Human ELISA Kit , Serum / Plasma Enzyme Linked Immunosorbent Assay Kit

Shanghai Korain Biotech Co., Ltd
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    Buy cheap Cystatin C Human ELISA Kit , Serum / Plasma Enzyme Linked Immunosorbent Assay Kit from wholesalers
     
    Buy cheap Cystatin C Human ELISA Kit , Serum / Plasma Enzyme Linked Immunosorbent Assay Kit from wholesalers
    • Buy cheap Cystatin C Human ELISA Kit , Serum / Plasma Enzyme Linked Immunosorbent Assay Kit from wholesalers

    Cystatin C Human ELISA Kit , Serum / Plasma Enzyme Linked Immunosorbent Assay Kit

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    Brand Name : BT Lab
    Model Number : Cat.No E0104Hu
    Certification : CE, ISO9001:2005, MSDS
    Price : Negotiation
    Supply Ability : Western Union, T/T
    Delivery Time : 1-3 business days, bulk order within one week
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    Cystatin C Human ELISA Kit , Serum / Plasma Enzyme Linked Immunosorbent Assay Kit

    96 wells Human Cystatin C CysC ELISA Kit Customized

    Cat.No E1104Hu
    Standard Curve Range: 0.05ng/ml - 20ng/ml
    Sensitivity: 0.028ng/ml
    Size: 96 wells
    Storage: Store the reagents at 2-8°C. For over 6-month storage refer to the expiration date keep it at -20°C. Avoid repeated thaw cycles. If individual reagents are opened it is recommended that the kit be used within 1 month.
    * This product is for research use only, not for use in diagnosis procedures. It’s highly recommend to read this instruction entirely before use.

    Precision
    Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
    Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
    CV(%) = SD/mean x 100
    Intra-Assay: CV<8%
    Inter-Assay: CV<10%

    Intended Use
    This sandwich kit is for the accurate quantitative detection of Human Cystatin C (also known as Cys-C) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

    Reagent Provided

    ComponentsQuantity
    Standard Solution (24ng/ml)0.5ml x1
    Pre-coated ELISA Plate12 * 8 well strips x1
    Standard Diluent3ml x1
    Streptavidin-HRP6ml x1
    Stop Solution6ml x1
    Substrate Solution A6ml x1
    Substrate Solution B6ml x1
    Wash Buffer Concentrate (30x)20ml x1
    Biotinylated Human Cys-C Antibody1ml x1
    User Instruction1
    Plate Sealer2 pics
    Zipper bag1 pic


    Reagent Preparation
    All reagents should be brought to room temperature before use.
    Standard Reconstitute the 120μl of the standard (24ng/ml) with 120μl of standard diluent to generate a 12ng/ml standard stock solution. Allow the standard to sit for 15 mins with gentle agitation prior to making dilutions. Prepare duplicate standard points by serially diluting the standard stock solution (12ng/ml) 1:2 with standard diluent to produce 6ng/ml, 3ng/ml, 1.5ng/ml and 0.75ng/ml solutions. Standard diluent serves as the zero standard(0 ng/ml). Any remaining solution should be frozen at -20°C and used within one month. Dilution of standard solutions suggested are as follows:

    12ng/mlStandard No.5120μl Original Standard + 120μl Standard Diluent
    6ng/mlStandard No.4120μl Standard No.5 + 120μl Standard Diluent
    3ng/mlStandard No.3120μl Standard No.4 + 120μl Standard Diluent
    1.5ng/mlStandard No.2120μl Standard No.3 + 120μl Standard Diluent
    0.75ng/mlStandard No.1120μl Standard No.2 + 120μl Standard Diluent

    Standard ConcentrationStandard No.5Standard No.4Standard No.3Standard No.2Standard No.1
    24ng/ml12ng/ml6ng/ml3ng/ml1.5ng/ml0.75ng/ml


    Wash Buffer Dilute 20ml of Wash Buffer Concentrate 30x into deionized or distilled water to yield 500 ml of 1x Wash Buffer. If crystals have formed in the concentrate, mix gently until the crystals have completely dissolved.

    Summary

    1. Prepare all reagents, samples and standards.

    2. Add sample and ELISA reagent into each well. Incubate for 1 hour at 37°C.

    3. Wash the plate 5 times.

    4. Add substrate solution A and B. Incubate for 10 minutes at 37°C.

    5. Add stop solution and color develops.

    6. Read the OD value within 10 minutes.

    References
    Janowski R., Kozak M., Abrahamson M., Grubb A., Jaskolski M.
    Proteins 61:570-578(2005)































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