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Shanghai Korain Biotech Co Ltd
8 Years
| Ask Lasest Price | |
| Brand Name : | BT Lab |
| Model Number : | Cat.No E2018Bo |
| Certification : | CE, ISO9001:2005, MSDS |
| Price : | Negotiation |
| Payment Terms : | Western Union, T/T |
| Supply Ability : | In Stock |
| Delivery Time : | 1-3 business days, bulk order within one week |
Bovine Fetuin A ELISA Kit with High Spesificity and Sensitivity
Cat.No E2018Bo
Reagent Provided
| Components | Quantity |
Standard Solution (1920mg/L) | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (30x) | 20ml x1 |
| Biotinylated Bovine FETU-A Antibody | 1ml x1 |
| User Instruction | 1 |
| Plate Sealer | 2 pics |
| Zipper bag | 1 pic |
Reagent Preparation
All reagents should be brought to room temperature before use.
Standard Reconstitute the 120μl of the standard (1920mg/L) with 120μl of standard diluent to generate a 960mg/L standard stock solution. Allow the standard to sit for 15 mins with gentle agitation prior to making dilutions. Prepare duplicate standard points by serially diluting the standard stock solution (960mg/L) 1:2 with standard diluent to produce 480mg/L, 240mg/L, 120mg/L and 60mg/L solutions. Standard diluent serves as the zero standard(0 mg/L). Any remaining solution should be frozen at -20℃ and used within one month. Dilution of standard solutions suggested are as follows:
| 960mg/L | Standard No.5 | 120μl Original Standard + 120μl Standard Diluent |
| 480mg/L | Standard No.4 | 120μl Standard No.5 + 120μl Standard Diluent |
| 240mg/L | Standard No.3 | 120μl Standard No.4 + 120μl Standard Diluent |
| 120mg/L | Standard No.2 | 120μl Standard No.3 + 120μl Standard Diluent |
| 60mg/L | Standard No.1 | 120μl Standard No.2 + 120μl Standard Diluent |
| Standard Concentration | Standard No.5 | Standard No.4 | Standard No.3 | Standard No.2 | Standard No.1 |
| 1920mg/L | 960mg/L | 480mg/L | 240mg/L | 120mg/L | 60mg/L |
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