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High Sensitive Mouse ELISA Kit for Activin A 96 wells Customized

Shanghai Korain Biotech Co., Ltd
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    Buy cheap High Sensitive Mouse ELISA Kit for Activin A 96 wells Customized from wholesalers
     
    Buy cheap High Sensitive Mouse ELISA Kit for Activin A 96 wells Customized from wholesalers
    • Buy cheap High Sensitive Mouse ELISA Kit for Activin A 96 wells Customized from wholesalers

    High Sensitive Mouse ELISA Kit for Activin A 96 wells Customized

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    Brand Name : BT Lab
    Model Number : Cat.No E0792Mo
    Certification : CE, ISO9001:2005, MSDS
    Price : Negotiation
    Payment Terms : Western Union, T/T
    Supply Ability : In Stock
    Delivery Time : 1-3 business days, bulk order within one week
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    High Sensitive Mouse ELISA Kit for Activin A 96 wells Customized

    High Sensitive Mouse ELISA Kit for Activin A 96 wells Customized​


    Storage: Store the reagents at 2-8°C. For over 6-month storage refer to the expiration date keep it at -20°C. Avoid repeated thaw cycles. If individual reagents are opened it is recommended that the kit be used within 1 month.

    *This product is for research use only, not for use in diagnosis procedures. It’s highly recommend to read this instruction entirely before use.


    Precision

    Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

    Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

    CV(%) = SD/mean x 100

    Intra-Assay: CV<8%

    Inter-Assay: CV<10%


    Assay Principle

    This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse ACV-A antibody. ACV-A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse ACV-A Antibody is added and binds to ACV-A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ACV-A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse ACV-A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.


    Reagent Provided

    ComponentsQuantity
    Standard Solution (3200ng/L)0.5ml x1
    Pre-coated ELISA Plate12 * 8 well strips x1
    Standard Diluent3ml x1
    Streptavidin-HRP6ml x1
    Stop Solution6ml x1
    Substrate Solution A6ml x1
    Substrate Solution B6ml x1
    Wash Buffer Concentrate (30x)20ml x1
    Biotinylated Mouse ACV-A Antibody1ml x1
    User Instruction1
    Plate Sealer2 pics
    Zipper bag1 pic

    Assay Procedure

    1. Prepare all reagents, standard solutions and samples as instructed. Bring all reagents to room temperature before use. The assay is performed at room temperature.

    2. Determine the number of strips required for the assay. Insert the strips in the frames for use. The unused strips should be stored at 2-8°C.

    3. Add 50μl standard to standard well. Note: Don’t add antibody to standard well because the standard solution contains biotinylated antibody.

    4. Add 40μl sample to sample wells and then add 10μl anti-ACV-A antibody to sample wells, then add 50μl streptavidin-HRP to sample wells and standard wells ( Not blank control well ). Mix well. Cover the plate with a sealer. Incubate 60 minutes at 37°C.

    5. Remove the sealer and wash the plate 5 times with wash buffer. Soak wells with at least 0.35 ml wash buffer for 30 seconds to 1 minute for each wash. For automated washing, aspirate all wells and wash 5 times with wash buffer, overfilling wells with wash buffer. Blot the plate onto paper towels or other absorbent material.

    6. Add 50μl substrate solution A to each well and then add 50μl substrate solution B to each well. Incubate plate covered with a new sealer for 10 minutes at 37°C in the dark.

    7. Add 50μl Stop Solution to each well, the blue color will change into yellow immediately.

    8. Determine the optical density (OD value) of each well immediately using a microplate reader set to 450 nm within 10 minuets after adding the stop solution.


    Summary

    1. Prepare all reagents, samples and standards.

    2. Add sample and ELISA reagent into each well. Incubate for 1 hour at 37°C.

    3. Wash the plate 5 times.

    4. Add substrate solution A and B. Incubate for 10 minutes at 37°C.

    5. Add stop solution and color develops.

    6. Read the OD value within 10 minutes.


    Cat.No E0792Mo
    Standard Curve Range: 10ng/L - 3000ng/L
    Sensitivity: 5.43ng/L
    Size: 96 wells

    Product Tags:

    sandwich elisa kit

      

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