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96 Wells Mouse Beta Defensin 4 ELISA Kit High Sensitive for Research Use

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    Buy cheap 96 Wells Mouse Beta Defensin 4 ELISA Kit High Sensitive for Research Use from wholesalers
     
    Buy cheap 96 Wells Mouse Beta Defensin 4 ELISA Kit High Sensitive for Research Use from wholesalers
    • Buy cheap 96 Wells Mouse Beta Defensin 4 ELISA Kit High Sensitive for Research Use from wholesalers

    96 Wells Mouse Beta Defensin 4 ELISA Kit High Sensitive for Research Use

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    Brand Name : BT Lab
    Model Number : Cat.No E1204Mo
    Certification : CE, ISO9001:2005, MSDS
    Price : Negotiation
    Payment Terms : Western Union, T/T
    Supply Ability : In Stock
    Delivery Time : 1-3 business days, bulk order within one week
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    96 Wells Mouse Beta Defensin 4 ELISA Kit High Sensitive for Research Use

    96 wells Mouse High Sensitivie Beta-defensin 4 ELISA Kit


    Cat.No E1204Mo

    Storage: Store the reagents at 2-8°C. For over 6-month storage refer to the expiration date keep it at -20°C. Avoid repeated thaw cycles. If individual reagents are opened it is recommended that the kit be used within 1 month.

    * This product is for research use only, not for use in diagnosis procedures. It’s highly recommend to read this instruction entirely before use.


    Assay Principle

    This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse DEFB4 antibody. DEFB4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse DEFB4 Antibody is added and binds to DEFB4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated DEFB4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse DEFB4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.


    Reagent Provided

    ComponentsQuantity
    Standard Solution (12.8ng/ml)0.5ml x1
    Pre-coated ELISA Plate12 * 8 well strips x1
    Standard Diluent3ml x1
    Streptavidin-HRP6ml x1
    Stop Solution6ml x1
    Substrate Solution A6ml x1
    Substrate Solution B6ml x1
    Wash Buffer Concentrate (30x)20ml x1
    Biotinylated Mouse DEFB4 Antibody1ml x1
    User Instruction1
    Plate Sealer2 pics
    Zipper bag1 pic

    Reagent Preparation

    All reagents should be brought to room temperature before use.

    Standard Reconstitute the 120μl of the standard (12.8ng/ml) with 120μl of standard diluent to generate a 6.4ng/ml standard stock solution. Allow the standard to sit for 15 mins with gentle agitation prior to making dilutions. Prepare duplicate standard points by serially diluting the standard stock solution (6.4ng/ml) 1:2 with standard diluent to produce 3.2ng/ml, 1.6ng/ml, 0.8ng/ml and 0.4ng/ml solutions. Standard diluent serves as the zero standard(0 ng/ml). Any remaining solution should be frozen at -20°C and used within one month. Dilution of standard solutions suggested are as follows:


    6.4ng/mlStandard No.5120μl Original Standard + 120μl Standard Diluent
    3.2ng/mlStandard No.4120μl Standard No.5 + 120μl Standard Diluent
    1.6ng/mlStandard No.3120μl Standard No.4 + 120μl Standard Diluent
    0.8ng/mlStandard No.2120μl Standard No.3 + 120μl Standard Diluent
    0.4ng/mlStandard No.1120μl Standard No.2 + 120μl Standard Diluent

    Standard ConcentrationStandard No.5Standard No.4Standard No.3Standard No.2Standard No.1
    12.8ng/ml6.4ng/ml3.2ng/ml1.6ng/ml0.8ng/ml0.4ng/ml

    Wash Buffer Dilute 20ml of Wash Buffer Concentrate 30x into deionized or distilled water to yield 500 ml of 1x Wash Buffer. If crystals have formed in the concentrate, mix gently until the crystals have completely dissolved.


    Summary

    1. Prepare all reagents, samples and standards.
    2. Add ample and ELISA reagent into each well. Incubate for 1 hour at 37°C.
    3. Wash the plate 5 times.

    4. Add substrate solution A and B. Incubate for 10 minutes at 37°C.

    5. Add stop solution and color develops.

    6. Read the OD value within 10 minutes.

    References

    Morrison G.M., Semple C.A.M., Kilanowski F.M., Hill R.E., Dorin J.R.
    Mol. Biol. Evol. 20:460-470(2003)


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